Long-Term In Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells
Christopher Kai-shun Leung, Robert N Weinreb, Zhi Wei Li, Shu Liu, James D Lindsey, Nathan Choi, Lan Liu, Carol Yim-lui Cheung, Cong Ye, Kunliang Qiu, Li Jia Chen, Wing Ho Yung, Jonathan G Crowston, Mingliang Pu, Kwok Fai So, Chi Pui Pang, Dennis Shun Chiu Lam
Investigative Ophthalmology and Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2011
PURPOSE: To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter. METHODS: A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degenerati..View full abstract
Supported by a CUHK research grant and the Glaucoma Foundation.