2D-PAGE of ovarian cancer: Analysis of soluble and insoluble fractions using medium-range immobilized pH gradients
Ratana Lim, Martha Lappas, Nuzhat Ahmed, Michael Permezel, Michael A Quinn, Gregory E Rice
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2011
Ovarian cancer remains a leading cause of cancer death. A comparative proteomic study was performed on normal ovarian tissue (n=5) and grade 3 ovarian tumours (n=5) to search for differentially expressed proteins. In contrast to other studies, here we extracted proteins in soluble and insoluble protein fractions using commercial kits and also utilised three medium-range IPG strips that encompassed the broad pH range of 3-10 (pH 3-6, 5-8 and 7-10). Protein fractions were compared by 2D-PAGE and MALDI-TOF/TOF-MS. Nineteen differentially expressed proteins were identified: HSP60, Grp78, CK19, EF-Tu, MRLC2, prohibitin, Stress-70 protein, TPI and tubulin α6 were up-regulated in grade 3 tumours wh..View full abstract
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Awarded by NHMRC
BOOTS (Breasts, Ovaries, and Other Things Sacred) and All Inc. provided funding for Ratana Lim. Professor Gregory Rice is a National Health and Medical Research Council (NHMRC) Principal Research Fellow. Dr. Martha Lappas is the recipient of a NHMRC RD Wright Fellowship (Grant No. 454777). The authors gratefully acknowledge the assistance of Julene Hallo of the Royal Women's Hospital for specimen collection and Karen Oliva (University of Queensland) for her expertise in mass spectrometry. The authors would also like to acknowledge the support of the Fight Cancer Foundation and Jigsaw.