Gene Sequencing and Tissue Expression of Unknown Isoforms of an Angiotensin II Type 2 Receptor Interacting Protein, ATIP, in the Rat
Michael Angus Krezel, Linda Adriana Rezmann, Naghmeh Varghayee, Josefa Pete, Albert George Frauman, Simon Nicholas Stewart Louis
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | TAYLOR & FRANCIS LTD | Published : 2011
To investigate the expression of the unknown angiotensin II type 2 receptor interacting protein (ATIP) isoforms in the rat we used the known sequences of human and mouse ATIP to design sequencing primers to enable us to sequence rat ATIP3 and ATIP4. Exon 4, which is present in human but not mouse ATIP, was not identified in the coding region of rat ATIP. The expression levels of these genes in a range of rat tissues were examined, and we concluded that there is little similarity in the relative tissue distribution of the various ATIP isoforms in rat and human.
Awarded by Kidney Health Australia
Awarded by Sir Edward Dunlop Medical Research Foundation
Awarded by Austin Hospital Medical Research Foundation
We thank Dr. Anthony Verberne and Dr. Daniela Sartor for providing SD rats for tissue harvesting. This work was supported by Kidney Health Australia (B 06 0809 to MAK, and 803969 to SNSL); the Sir Edward Dunlop Medical Research Foundation (809280 to SNSL); and the Austin Hospital Medical Research Foundation (2-1377 to SNSL).