Deerpox Virus Encodes an Inhibitor of Apoptosis That Regulates Bak and Bax
Logan Banadyga, Sing-Chi Lam, Toru Okamoto, Marc Kvansakul, David C Huang, Michele Barry
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2011
Poxviruses encode numerous proteins that inhibit apoptosis, a form of cell death critical to the elimination of virally infected cells. Sequencing of the deerpox virus genome revealed DPV022, a protein that lacks obvious homology to cellular members of the Bcl-2 family but shares limited regions of amino acid identity with two unique poxviral inhibitors of apoptosis, M11L and F1L. Given the limited homology, we sought to determine whether DPV022 could inhibit apoptosis. Here we show that DPV022 localized to the mitochondria, where it inhibited apoptosis. We used a Saccharomyces cerevisiae model system to demonstrate that in the absence of all other Bcl-2 family proteins, DPV022 interacted di..View full abstract
Awarded by Australian National Health and Medical Research Council
Awarded by Leukemia and Lymphoma Society
This research was supported by grants and fellowships from the Canadian Institutes of Health Research, the Howard Hughes Medical Institute, and Alberta Innovates-Health Solutions to M.B.; the Australian National Health and Medical Research Council (program grant 461221, project grant 637336, fellowships to M.K. and D.C.H., and IRIISS grant 361646); the Australian Research Council (fellowship to T.O.); the Leukemia and Lymphoma Society (SCOR grant 7413-07); the Australian Cancer Research Foundation; and the Victorian State Government (OIS grant). L. B. was supported by a Canada graduate doctoral scholarship from the National Sciences and Engineering Council of Canada (NSERC-CGSD) and an Alberta Innovates-Health Solutions studentship. M.B. is a tier I Canada research chair, a senior scholar of Alberta Innovates-Health Solutions, and a Howard Hughes Medical Institute scholar in infection and parasitology.