Journal article
ERG dependence distinguishes developmental control of hematopoietic stem cell maintenance from hematopoietic specification
S Taoudi, T Bee, A Hilton, K Knezevic, J Scott, TA Willson, C Collin, T Thomas, AK Voss, BT Kile, WS Alexander, JE Pimanda, DJ Hilton
Genes and Development | Published : 2011
DOI: 10.1101/gad.2009211
Abstract
Although many genes are known to be critical for early hematopoiesis in the embryo, it remains unclear whether distinct regulatory pathways exist to control hematopoietic specification versus hematopoietic stem cell (HSC) emergence and function. Due to their interaction with key regulators of hematopoietic commitment, particular interest has focused on the role of the ETS family of transcription factors; of these, ERG is predicted to play an important role in the initiation of hematopoiesis, yet we do not know if or when ERG is required. Using in vitro and in vivo models of hematopoiesis and HSC development, we provide strong evidence that ERG is at the center of a distinct regulatory progra..
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Awarded by Australian National Health and Medical Research Council of Australia (NHMRC)
Funding Acknowledgements
We thank Dr. Ashley Ng for practical assistance and critical discussions, and WEHI services for assistance with animal husbandry, cell sorting, mouse irradiation, and histological preparation of samples. We also thank Dr. Julie Sheridan and Dr. Christele Gonneau for critical comments on the manuscript. This work was supported by a Program grant (461219), Project grant (516726, 510100, 568668), Fellowships (to D.J.H., W.S.A., A.K.V., and B.T.K.) and Independent Research Institutes Support Scheme grant (361646) from the Australian National Health and Medical Research Council of Australia (NHMRC); the Australian Cancer Research Foundation; Australian Stem Cell Centre collaborative stream grant (adult stem cells, module 8); a Fellowship from the Australian Research Council (to B.T.K.); a Fellowship from the Sylvia and Charles Viertel Charitable Foundation (to B.T.K.); and a Victorian State Government Operational Infrastructure Support grant.