Characterisation of HvALMT1 function in transgenic barley plants
Benjamin D Gruber, Emmanuel Delhaize, Alan E Richardson, Ute Roessner, Richard A James, Susan M Howitt, Peter R Ryan
Functional Plant Biology - an international journal of plant function | CSIRO PUBLISHING | Published : 2011
HvALMT1 from barley (Hordeum vulgare L.) encodes a protein capable of facilitating the transport of malate and other organic anions when expressed in Xenopus oocytes. The HvALMT1 gene is primarily expressed in guard cells of stomata, in regions behind the root apex and at lateral root junctions. We investigated the function of HvALMT1 in planta by overexpressing it in barley under the control of a constitutive promoter. Transgenic plants expressing HvALMT1 at levels four to 9-fold greater than controls showed reduced growth and plants showing the highest expression failed to set seed. Although measurements of conductance indicated that stomatal function was not totally impaired in the transg..View full abstract
Thanks to Alec Zwart for statistical advice relating to the layout of plants for the metabolomics experiments and to CSIRO Analytical Services for conducting the elemental analysis. Thanks also to Diane Hebb for preparing the constructs and Agrobacteria used for the transformation of barley and Arabidopsis with HvALMT1. Support to BG was provided by the Grains Research and Development Corporation of Australia.