Journal article
A proteomic analysis of C-reactive protein stimulated THP-1 monocytes
SU Eisenhardt, J Habersberger, K Oliva, GI Lancaster, M Ayhan, KJ Woollard, H Bannasch, GE Rice, K Peter
Proteome Science | BMC | Published : 2011
Abstract
Background: C-reactive protein (CRP) is a predictor of cardiovascular risk. It circulates as a pentameric protein in plasma. Recently, a potential dissociation mechanism from the disc-shaped pentameric CRP (pCRP) into single monomers (monomeric or mCRP) has been described. It has been shown that mCRP has strong pro-inflammatory effects on monocytes. To further define the role of mCRP in determining monocyte phenotype, the effects of CRP isoforms on THP-1 protein expression profiles were determined. The hypothesis to be tested was that mCRP induces specific changes in the protein expression profile of THP-1 cells that differ from that of pCRP.Methods: Protein cell lysates from control and mCR..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
SUE was supported by a National Health and Medical Research Council (NHMRC) project grant # 472666, and a grant from the German Research Foundation (DFG) #EI 866/1-1, JH is in receipt of a combined scholarship of the NHMRC and the National Heart Foundation of Australia # 586651, KO is in receipt of a grant of the Heart Foundation of Australia, GER is a Principal Research Fellow of the NHMRC and the work was supported by the NHMRC Project Grant # 526686. KP is a future fellow of the Australian Research Council.