Distinct roles in NKT cell maturation and function for the different transcription factors in the classical NF-kappa B pathway
Sanda Stankovic, Raffi Gugasyan, Konstantinos Kyparissoudis, Raelene Grumont, Ashish Banerjee, Philip Tsichlis, Steve Gerondakis, Dale I Godfrey
Immunology and Cell Biology | WILEY | Published : 2011
The nuclear factor (NF)-κB signalling pathway is known to be critical for natural killer T (NKT) cell differentiation; however, the role of individual NF-κB transcription factors and the precise developmental stages that they control remain unclear. We have investigated the influence of the classical NF-κB transcription factors NF-κB1, c-Rel and RelA on NKT cell development and function, using gene-deleted mice. Individually, none of these factors were essential for the requirement of NF-κB signalling in early NKT cell development before NK1.1 expression, in contrast to earlier reports in which the classical NF-κB pathway was globally disrupted. Instead, we found that each factor played a no..View full abstract
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Awarded by NHMRC
Awarded by Leukemia and Lymphoma Society (SCOR)
This work was supported by an NHMRC Program Grant (454569) to DIG, plus Leukemia and Lymphoma Society (SCOR Grant 7015) and NHMRC (257502) Program grants to SG. SG and DIG are supported by NHMRC Research Fellowships. SS is supported by ECR and CASS Foundation grants. We thank Professor Andreas Strasser (WEHI) for his generous support of this work and acknowledge Mr Ken Field for assistance with flow cytometry and the Picchi Brothers Foundation for their generous contribution to the University of Melbourne, Department of Microbiology and Immunology flow cytometry facility.