Journal article
Enhancing the specificity of T-cell cultures for adoptive immunotherapy of cancer
CP Duong, JA Westwood, LJ Berry, PK Darcy, MH Kershaw
Immunotherapy | Published : 2011
DOI: 10.2217/imt.10.81
Abstract
Adoptive immunotherapy is a promising approach for the treatment of cancer; however, autoimmunity against normal tissue can be a serious complication of this therapy. We hypothesized that T-cell cultures responding maximally only when engaging two antigens would be more specific for tumor cells, and less active against normal cells, as long as the tumor expressed both antigens, while normal cells expressed only one of the antigens. A model system was developed consisting of cell lines expressing either folate binding protein or erbB-2, representing 'normal tissue, and cells expressing both antigens representing tumor tissue. Human T-cell cultures were produced using two chimeric antigen rece..
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Funding Acknowledgements
This work was supported by grants from the National Health and Medical Research Council of Australia (NHMRC), the Cancer Council of Victoria, the Susan G Kamen Breast Cancer Foundation, the Bob Parker Memorial Trust and the Peter MacCallum Cancer Centre Foundation. Michael Kershaw and Phillip Darcy are supported by a Senior Research Fellowship and Career Development Awards from the NHMRC. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.