Journal article

Temporal Investigation of Matrix Metalloproteinases and Their Inhibitors in Human Cervicovaginal Fluid in Late Pregnancy and Labor

Yujing J Heng, Megan KW Di Quinzio, Stella Liong, Michael Permezel, Gregory E Rice, Harry M Georgiou



Temporal expression of matrix metalloproteinase (MMP)-1, -2, -3, -7, -8, -9, -12, and -13, and tissue inhibitors of metalloproteinases (TIMPs)-1 and -2 in human cervicovaginal fluid (CVF) in term pregnancy and labor was investigated. Term parous women provided CVF samples that were grouped into labor, 1 to 3, 6 to 8, and 12 to 16 days before labor onset. Both MMPs and TIMPs (n = 60) were quantified using multiplex solution array and enzyme-linked immunosorbent assays, respectively. Further analysis of TIMP-1 (n = 180) was undertaken. All MMPs and TIMPs except MMP-12 and -13 were detected in the CVF. Matrix metalloproteinase 7, TIMP-1, and TIMP-2 were significantly increased in labor. Tissue ..

View full abstract


Awarded by National Health and Medical Research Council of Australia (NHMRC)

Awarded by NHMRC

Funding Acknowledgements

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: National Health and Medical Research Council of Australia (NHMRC) Development Grant (454451); Medical Research Foundation for Women and Babies; Y.J.H. is a recipient of the NHMRC Biomedical (Dora Lush) postgraduate research scholarship (454880).