Temporal Investigation of Matrix Metalloproteinases and Their Inhibitors in Human Cervicovaginal Fluid in Late Pregnancy and Labor
Yujing J Heng, Megan KW Di Quinzio, Stella Liong, Michael Permezel, Gregory E Rice, Harry M Georgiou
REPRODUCTIVE SCIENCES | SAGE PUBLICATIONS INC | Published : 2012
Temporal expression of matrix metalloproteinase (MMP)-1, -2, -3, -7, -8, -9, -12, and -13, and tissue inhibitors of metalloproteinases (TIMPs)-1 and -2 in human cervicovaginal fluid (CVF) in term pregnancy and labor was investigated. Term parous women provided CVF samples that were grouped into labor, 1 to 3, 6 to 8, and 12 to 16 days before labor onset. Both MMPs and TIMPs (n = 60) were quantified using multiplex solution array and enzyme-linked immunosorbent assays, respectively. Further analysis of TIMP-1 (n = 180) was undertaken. All MMPs and TIMPs except MMP-12 and -13 were detected in the CVF. Matrix metalloproteinase 7, TIMP-1, and TIMP-2 were significantly increased in labor. Tissue ..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by NHMRC
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: National Health and Medical Research Council of Australia (NHMRC) Development Grant (454451); Medical Research Foundation for Women and Babies; Y.J.H. is a recipient of the NHMRC Biomedical (Dora Lush) postgraduate research scholarship (454880).