Journal article
Preparation and biological evaluation of self-assembled cubic phases for the polyvalent inhibition of cholera toxin
SJ Fraser, R Rose, MK Hattarki, PG Hartley, O Dolezal, RM Dawson, F Separovic, A Polyzos
Soft Matter | ROYAL SOC CHEMISTRY | Published : 2011
DOI: 10.1039/c1sm05428g
Abstract
The inverse cubic phase derived from the self-assembly of surfactants in water offers a unique three-dimensional platform for protein binding. Colloidally stable, sub-micron dispersions of the inverse bicontinuous cubic phase (cubosomes) impart an unusually large interfacial area for presentation of small molecules to selectively bind proteins of interest. Cubosomes of the phytantriol/water system were prepared and the receptor for cholera toxin (CT), monosialoganglioside GM1 (GM1), was integrated within the cubic phase. Our results show that GM1-functionalised cubosomes display a strong inhibitory response against CT with a high specificity for the toxin. Surface plasmon resonance (SPR) stu..
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Funding Acknowledgements
The authors are grateful to the Office of the Chief Executive (CSIRO) for PhD Studentship funding (SJF) and the Australian Synchrotron for SAXS beamline allocation. The views expressed herein are those of the authors and are not necessarily those of the owner or operator of the Australian Synchrotron. We thank beamline scientists, Nigel Kirby and Stephen Mudie, for their assistance during data collection. The contributions of Lynne Waddington (CSIRO) for providing Cryo-TEM images and Dr Xavier Mulet (CSIRO) for his assistance in the preparation of graphical material are greatly appreciated.