Effects of ultrasound on the thermal and structural characteristics of proteins in reconstituted whey protein concentrate
Jayani Chandrapala, Bogdan Zisu, Martin Palmer, Sandra Kentish, Muthupandian Ashokkumar
Ultrasonics Sonochemistry | ELSEVIER SCIENCE BV | Published : 2011
The sonication-induced changes in the structural and thermal properties of proteins in reconstituted whey protein concentrate (WPC) solutions were examined. Differential scanning calorimetry, UV-vis, fluorescence and circular dichroism spectroscopic techniques were used to determine the thermal properties of proteins, measure thiol groups and monitor changes to protein hydrophobicity and secondary structure, respectively. The enthalpy of denaturation decreased when WPC solutions were sonicated for up to 5 min. Prolonged sonication increased the enthalpy of denaturation due to protein aggregation. Sonication did not alter the thiol content but resulted in minor changes to the secondary struct..View full abstract
This study was supported by an Australian Research Council Linkage Project and Dairy Innovation, Australia Ltd.