Journal article
Controlled release of DNA from poly(vinylpyrrolidone) capsules using cleavable linkers
SL Ng, GK Such, APR Johnston, G Antequera-García, F Caruso
Biomaterials | ELSEVIER SCI LTD | Published : 2011
Abstract
The design of polymer carriers with tunable degradation and cargo release is fundamental for applications in drug and gene delivery. In this study, we report low-fouling poly(N-vinyl pyrrolidone) (PVPON) capsules assembled via hydrogen bonding and stabilized using covalent cross-linking. We first investigated the effects of pH and ionic strength to optimize the assembly conditions. A model therapeutic cargo (plasmid DNA) was then loaded in the capsules and used for encapsulation and release studies. Two bisazide cross-linkers that contain a disulfide bond, termed PEG 8 (poly(ethylene glycol)) and PEG 16, were employed to stabilize the multilayer films, and used to tune the degradation and ca..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by the Australian Research Council under the Federation Fellowship (F.C.) and Discovery Project schemes (F.C., A.P.R.J. and G.K.S.). G.A.-G. acknowledges the financial support from Consolider-Ingenio Nanobiomed (Spanish MEC, Spain) and Prof. Liz-Marzan (University of Vigo) for supporting her research stay at The University of Melbourne. Dr. Kim Wark (CSIRO) is acknowledged for providing the plasmid.