Journal article
The scutellar vascular bundle–specific promoter of the wheat HD-Zip IV transcription factor shows similar spatial and temporal activity in transgenic wheat, barley and rice
NK Kovalchuk, WW Wu, OE Eini, NB Bazanova, MP Pallotta, NS Shirley, RS Singh, AI Ismagul, ES Eliby, AAT Johnson, PL Langridge, SL Lopato
Plant Biotechnology Journal | Blackwell Publishing Ltd | Published : 2012
Abstract
An HD-Zip IV gene from wheat, TaGL9, was isolated using a Y1H screen of a cDNA library prepared from developing wheat grain. TaGL9 has an amino acid sequence distinct from other reported members of the HD-Zip IV family. The 3' untranslated region of TaGL9 was used as a probe to isolate a genomic clone of the TaGL9 homologue from a BAC library prepared from Triticum durum L. cv. Langdon. The full-length gene containing a 3-kb-long promoter region was designated TdGL9H1. Spatial and temporal activity of TdGL9H1 was examined using promoter-GUS fusion constructs in transgenic wheat, barley and rice plants. Whole-mount and histochemical GUS staining patterns revealed grain-specific expression of ..
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Funding Acknowledgements
We thank Anita Lapina for technical assistance with BAC DNA isolation and promoter cloning, Ursula Langridge, Lorraine Carruthers and Alex Kovalchuk for assistance with growing plants in the glasshouse and John Harris for critical reading of the manuscript. This work was supported by the Australian Research Council, the Grains Research and Development Corporation and the Government of South Australia.