Targeting Antigen to Mouse Dendritic Cells via Clec9A Induces Potent CD4 T Cell Responses Biased toward a Follicular Helper Phenotype
Mireille H Lahoud, Fatma Ahmet, Susie Kitsoulis, Soo San Wan, David Vremec, Chin-Nien Lee, Belinda Phipson, Wei Shi, Gordon K Smyth, Andrew M Lew, Yu Kato, Scott N Mueller, Gayle M Davey, William R Heath, Ken Shortman, Irina Caminschi
Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 2011
Three surface molecules of mouse CD8(+) dendritic cells (DCs), also found on the equivalent human DC subpopulation, were compared as targets for Ab-mediated delivery of Ags, a developing strategy for vaccination. For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants. For Ab production, however, Clec9A excelled as a target, even in the absence of adjuvant. Potent humoral immunity was a result of the highly specific expression of Clec9A on DCs, which allowed longer residence of targeting Abs in the bloodstream, prolonged DC Ag presentation, and extended CD4 T cell proliferation, all of which drove highly..View full abstract
This work was supported by the National Health and Medical Research Council of Australia.