Journal article
Skeletal recovery after weaning does not require PTHrP
BJ Kirby, L Ardeshirpour, JP Woodrow, JJ Wysolmerski, NA Sims, AC Karaplis, CS Kovacs
Journal of Bone and Mineral Research | Published : 2011
DOI: 10.1002/jbmr.339
Abstract
Mice lose 20% to 25% of trabecular bone mineral content (BMC) during lactation and restore it after weaning through unknown mechanisms. We found that tibial Pthrp mRNA expression was upregulated fivefold by 7 days after weaning versus end of lactation in wild-type (WT) mice. To determine whether parathyroid hormone-related protein (PTHrP) stimulates bone formation after weaning, we studied a conditional knockout in which PTHrP is deleted from preosteoblasts and osteoblasts by collagen I promoter-driven Cre (CreColI). These mice are osteopenic as adults but have normal serum calcium, calcitriol, and parathyroid hormone (PTH). Pairs of Pthrpflox/flox;CreColI (null) and WT;CreColI (WT) females ..
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Awarded by National Cancer Institute
Funding Acknowledgements
We thank Ingrid Poulton for prompt and high-quality histology and histomorphometry. This work was supported by operating grants to CSK from the Canadian Institutes of Health Research, to JJW from the National Institutes of Health, and to NAS from the National Health and Medical Research Council (Australia).