Glucose consumption of single post-compaction human embryos is predictive of embryo sex and live birth outcome

Abstract

Background: The aim of this study was to determine the relationship between nutrient utilization by the human embryo and its subsequent viability after transfer. Methods: The embryos of 50 patients having single blastocyst transfer were cultured individually from Day 3 in 10 μl drops of medium G2 under Ovoil in 5%O2, 6%CO2, 89%N2. Patient inclusion in the study was maternal age ≤38. Embryos were moved to fresh drops of medium every 24 h. Spent media samples, including controls containing no embryo, were coded, frozen and subsequently analysed blind. Analysis of glucose was performed by microfluorimetry. The sex of children born was recorded. Results: Clinical pregnancy and live birth rates w..