Prolonged Xenograft Survival Induced by Inducible Costimulator-Ig Is Associated With Increased Forkhead Box P3( ) Cells
Russell Hodgson, Dale Christiansen, Andrew Ziolkowski, Effie Mouhtouris, Charmaine J Simeonovic, Francesco L Ierino, Mauro S Sandrin
TRANSPLANTATION | LIPPINCOTT WILLIAMS & WILKINS | Published : 2011
BACKGROUND: Blockade of the inducible costimulator (ICOS) pathway has been shown to prolong allograft survival; however, its utility in xenotransplantation is unknown. We hypothesize that local expression of ICOS-Ig by the xenograft will suppress the T-cell response resulting in significant prolonged graft survival. METHODS: Pig iliac artery endothelial cells (PIEC) secreting ICOS-Ig were generated and examined for the following: (1) inhibition of allogeneic and xenogeneic proliferation of primed T cells in vitro and (2) prolongation of xenograft survival in vivo. Grafts were examined for Tregs by flow cytometry and cytokine levels determined by quantitative reverse-transcriptase polymerase ..View full abstract
This work was supported, in part, by the National Health and Medical Research Council of Australia.