First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia

YAL Lim; A Iqbal; J Surin; BLH Sim; AR Jex; MJ Nolan; HV Smith; RB Gasser

Journal article

First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia

YAL Lim, A Iqbal, J Surin, BLH Sim, AR Jex, MJ Nolan, HV Smith, RB Gasser

Infection Genetics and Evolution | ELSEVIER SCIENCE BV | Published : 2011

DOI: 10.1016/j.meegid.2011.03.007

Abstract

Given the HIV epidemic in Malaysia, genetic information on opportunistic pathogens, such as Cryptosporidium and Giardia, in HIV/AIDS patients is pivotal to enhance our understanding of epidemiology, patient care, management and disease surveillance. In the present study, 122 faecal samples from HIV/AIDS patients were examined for the presence of Cryptosporidium oocysts and Giardia cysts using a conventional coproscopic approach. Such oocysts and cysts were detected in 22.1% and 5.7% of the 122 faecal samples, respectively. Genomic DNAs from selected samples were tested in a nested-PCR, targeting regions of the small subunit (SSU) of nuclear ribosomal RNA and the 60. kDa glycoprotein (gp60) g..

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University of Melbourne Researchers

Aaron Jex Author

Robin B. Gasser Author

Grants

Funding Acknowledgements

We thank Mohammed Mahdy (University of Malaya), Rosely Nichols, Lisa Connelly (Scottish Parasite Diagnostic Laboratory) and Ross Hall (Gasser Lab) for their assistance. Current research in the Gasser Lab is funded mainly by the Australian Research Council (ARC), the National Health & Medical Research Council (NHMRC) and Melbourne Water Corporation.