Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections
Florian Roeber, Aaron R Jex, Angus JD Campbell, Bronwyn E Campbell, Garry A Anderson, Robin B Gasser
INFECTION GENETICS AND EVOLUTION | ELSEVIER | Published : 2011
We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep (n = 470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 'negative control' samples from sheep known not to harbour parasitic helminths. We compared the diagnostic sensitivity (98%) and specificity (100%) of this assay against a conventional faecal flotation method and also established a system to rank the contribution of particular strongylid nematodes to the faecal egg counts (FECs) from 'mixed infections' in individual sheep. The testing of faecal samples herein revealed that Teladorsagia circumcincta (80%) and Trichostrongylus spp. (..View full abstract
Funding from the Australian Research Council is gratefully acknowledged (RBG). Thanks to Ian Beveridge, John Larsen, Norman Anderson, Dianne Rees, Christine Andersen, Neil Young, Matt Nolan and Bjoern Schaeffner for discussions and/or support. FR is the grateful recipient of scholarships from The University of Melbourne.