Journal article

Differentiation-dependent functional and epigenetic landscapes for cytokine genes in virus-specific CD8( ) T cells

Alice E Denton, Brendan E Russ, Peter C Doherty, Sudha Rao, Stephen J Turner

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2011

Abstract

Although the simultaneous engagement of multiple effector mechanisms is thought to characterize optimal CD8(+) T-cell immunity and facilitate pathogen clearance, the differentiation pathways leading to the acquisition and maintenance of such polyfunctional activity are not well understood. Division-dependent profiles of effector molecule expression for virus-specific T cells are analyzed here by using a combination of carboxyfluorescein succinimidyl ester dilution and intracellular cytokine staining subsequent to T-cell receptor ligation. The experiments show that, although the majority of naive CD8(+) T-cell precursors are preprogrammed to produce TNF-α soon after stimulation and a proporti..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by Australian National Health and Medical Research Council (NHMRC) Program Grant 5671222 (to P.C.D. and S.J.T.), NHMRC Project Grant 1003131 (to S.J.T. and S.R.), National Institutes of Health Grant AI70251 (to P.C.D.), an NHMRC Dora Lush Postgraduate Award (to A.E.D.), and a Pfizer Senior Research Fellowship (to S.J.T.); and by the American Lebanese Syrian Associated Charities.