Biomaterials for Spinal Cord Regeneration: Outgrowth of Presumptive Neuronal Precursors on Electrospun Poly(epsilon)-caprolactone Scaffolds Microlayered with Alternating Polyelectrolytes
George A Thouas, Karla G Contreras, Claude C Bernard, Gui-Zhi Sun, Kelly Tsang, Kun Zhou, David R Nisbet, John S Forsythe
2008 30TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-8 | IEEE | Published : 2008
The aim of this study was to assess the feasibility of electrospun poly(epsilon)-caprolactone (PCL) scaffolds treated with alternating paly-electrolytes as a controllable three-dimensional adhesive substrate for neuronal progenitors. Unmodified PCL surfaces were generally not supportive of mouse embryonic stem cell (mESC) colony adhesion. However, scaffolds surfaced using layer-by-layer (LbL) deposition of heparin/poly-L-lysine encouraged better local adhesion of mESC colonies, and networking of monolayers containing nestin-positive presumptive neurons, similar to laminin coated controls, as observed by immuno-fluorescence microscopy. Confocal microscopy further revealed depth-wise penetrati..View full abstract
This work was supported in part by gants from the NHMRC, the Baker Foundation of Australia, and the National Multiple Sclerosis Society of New York.