Journal article
Integration of EST-SSR markers of Medicago truncatula into intraspecific linkage map of lentil and identification of QTL conferring resistance to ascochyta blight at seedling and pod stages
D Gupta, PWJ Taylor, P Inder, HTT Phan, SR Ellwood, PN Mathur, A Sarker, R Ford
Molecular Breeding | Published : 2012
Abstract
Microsatellite markers have been extensively utilised in the leguminosae for genome mapping and identifying major loci governing traits of interest for eventual marker-assisted selection (MAS). The lack of available lentil-specific microsatellite sequences and gene-based markers instigated the mining and transfer of expressed sequence tag simple sequence repeat (EST-SSR)/SSR sequences from the model genome Medicago truncatula, to enrich an existing intraspecific lentil genetic map. A total of 196 markers, including new 15 M. truncatula EST-SSR/SSR, were mapped using a population of 94 F5 recombinant inbred lines produced from a cross between cv. Northfield (ILL5588) × cv. Digger (ILL5722) an..
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Funding Acknowledgements
We wish to thank Bioversity International, Rome, for awarding the Vavilov-Frankel Fellowship to the first author in order to support this work at the University of Melbourne. We are grateful to Dr Michael Materne of the Victorian Department of Primary Industries (VicDPI), Horsham, Victoria, Australia for providing seed and breeding advice.