Journal article

Klf4 Interacts Directly with Oct4 and Sox2 to Promote Reprogramming

Zong Wei, Yang Yang, Peilin Zhang, Rosemary Andrianakos, Kouichi Hasegawa, Jungmook Lyu, Xi Chen, Gang Bai, Chunming Liu, Martin Pera, Wange Lu

STEM CELLS | WILEY | Published : 2009

Abstract

Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by ectopic expression of specific sets of transcription factors. Oct4, Sox2, and Klf4, factors that share many target genes in embryonic stem (ES) cells, are critical components in various reprogramming protocols. Nevertheless, it remains unclear whether these factors function together or separately in reprogramming. Here we show that Klf4 interacts directly with Oct4 and Sox2 when expressed at levels sufficient to induce iPS cells. Endogenous Klf4 also interacts with Oct4 and Sox2 in iPS cells and in mouse ES cells. The Klf4 C terminus, which contains three tandem zinc fingers, is critical for this interaction and is ..

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University of Melbourne Researchers