Journal article

Derivation of neural precursors from human embryonic stem cells in the presence of noggin

P Itsykson, N Ilouz, T Turetsky, RS Goldstein, MF Pera, I Fishbein, M Segal, BE Reubinoff

MOLECULAR AND CELLULAR NEUROSCIENCE | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2005

Abstract

The utilization of human embryonic stem cells (hESC) for basic and applied research is hampered by limitations in directing their differentiation. Empirical poorly defined methods are currently used to develop cultures enriched for distinct cell types. Here, we report the derivation of neural precursors (NPs) from hESC in a defined culture system that includes the bone morphogenetic protein antagonist noggin. When hESC are cultured as floating aggregates in defined medium and BMP signaling is repressed by noggin, non-neural differentiation is suppressed, and the cell aggregates develop into spheres highly enriched for proliferating NPs. The NPs can differentiate into astrocytes, oligodendroc..

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University of Melbourne Researchers