Bacterial expression and purification of active hematopoietic cell kinase
Romana Kristelly, Theresa Wenli Qiu, Natalie J Gunn, Denis B Scanlon, Terrence D Mulhern
Protein Expression and Purification | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2011
Awarded by National Health and Medical Research Council
We are indebted to Heung-Chin Cheng and John Kuriyan for the provision of the pcDNA3 Hck and YopH plasmids, respectively. We thank Daisy Sio Seng Lio for technical assistance and Nicholas Williamson for assistance with the analysis of the mass spectrometry data. This work was supported by a University of Melbourne ANSTO Collaborative Grant (RK and TDM) and by the National Health and Medical Research Council Project Grant 509115 (TDM).