Journal article
Comprehensive profiling and quantitation of amine group containing metabolites.
BA Boughton, DL Callahan, C Silva, J Bowne, A Nahid, T Rupasinghe, DL Tull, MJ McConville, A Bacic, U Roessner
Analytical Chemistry | AMER CHEMICAL SOC | Published : 2011
DOI: 10.1021/ac201610x
Abstract
Primary and secondary amines, including amino acids, biogenic amines, hormones, neurotransmitters, and plant siderophores, are readily derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using easily performed experimental methodology. Complex mixtures of these amine derivatives can be fractionated and quantified using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Upon collision induced dissociation (CID) in a quadrupole collision cell, all derivatized compounds lose the aminoquinoline tag. With the use of untargeted fragmentation scan functions, such as precursor ion scanning, the loss of the aminoquinoline tag (Amq) can be monitored to identify d..
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Funding Acknowledgements
B.A.B. and D.L.C. contributed equally to this work. The authors are grateful to the Victorian Node of Metabolomics Australia, which is funded through Bioplatforms Australia Pty Ltd, a National Collaborative Research Infrastructure Strategy (NCRIS), 5.1 biomolecular platforms and informatics investment, and coinvestment from the Victorian State government and The University of Melbourne. U.R. thanks the Australian Centre for Plant Functional Genomics (ACPFG), which is funded by grants from the Australian Research Council (ARC) and the Grains Research and Development Corporation (GRDC), the South Australian Government, and the University of Adelaide, the University of Queensland, and The University of Melbourne. A.B. acknowledges the support of the ARC Centre of Excellence in Plant Cell Walls. M.J.M. is a NHMRC Principal Research Fellow. We thank Mr. David de Souza and Dr. Eleanor Saunders for providing the Leishmania mexicana cell samples.