Journal article

MicroRNAs in the shoot apical meristem of soybean

Chui E Wong, Ying-Tao Zhao, Xiu-Jie Wang, Larry Croft, Zhong-Hua Wang, Farzad Haerizadeh, John S Mattick, Mohan B Singh, Bernard J Carroll, Prem L Bhalla

JOURNAL OF EXPERIMENTAL BOTANY | OXFORD UNIV PRESS | Published : 2011

Abstract

Plant microRNAs (miRNAs) play crucial regulatory roles in various developmental processes. In this study, we characterize the miRNA profile of the shoot apical meristem (SAM) of an important legume crop, soybean, by integrating high-throughput sequencing data with miRNA microarray analysis. A total of 8423 non-redundant sRNAs were obtained from two libraries derived from micro-dissected SAM or mature leaf tissue. Sequence analysis allowed the identification of 32 conserved miRNA families as well as 8 putative novel miRNAs. Subsequent miRNA profiling with microarrays verified the expression of the majority of these conserved and novel miRNAs. It is noteworthy that several miRNAs* were express..

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