Border sequences of Medicago truncatula CLE36 are specifically cleaved by endoproteases common to the extracellular fluids of Medicago and soybean
Michael A Djordjevic, Marie Oakes, Chui E Wong, Mohan Singh, Prem Bhalla, Lucia Kusumawati, Nijat Imin
Journal of Experimental Botany | OXFORD UNIV PRESS | Published : 2011
CLE (CLAVATA3/ESR-related) peptides are developmental regulators that are secreted into the apoplast. Little is known about the role of the sequences that flank CLE peptides in terms of their biological activity or how they are targeted by proteases that are known to liberate the final active CLE peptides from their precursor sequences. The biological activity of Medicago truncatula CLE36, which possesses broadly conserved border sequences flanking the putative final active CLE36 peptide product, was assessed. Using in vitro root growth assays and an in vitro root and callus formation assay it is shown that CLE36 peptides of different lengths possess differential biological activities. Using..View full abstract
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Awarded by Australian Research Council
We acknowledge our colleagues at the Australian Research Council Centre of Excellence for Integrative Legume Research (CILR), especially Karsten Oelkers, Jeremy Weinman, Ulrike Mathesius, and Peter Gresshoff for fruitful discussions. LK is grateful for financial support from the AUSAid Scheme. We acknowledge Isabel Saur for assistance with the tissue culture experiments. Angelia M. Hartono is acknowledged for assistance with root growth inhibition assays. Dr Peter Milburn of the Biomolecular Resource Facility at the ANU is thanked for operating the MALDI-TOF/TOF. ARA-12 antibody was supplied by Dr John Hamilton, School of Biological and Biomedical Sciences, University of Durham, Durham UK. This research was funded by the Australian Research Council's Centre of Excellence Scheme (CE0348212).