Journal article

Differential expression of oxygen-regulated genes in bovine blastocysts

AJ Harvey, A Navarrete Santos, M Kirstein, KL Kind, B Fischer, JG Thompson

Molecular Reproduction and Development | WILEY-LISS | Published : 2007


Low oxygen conditions (2%) during post-compaction culture of bovine blastocysts improve embryo quality, which is associated with a small yet significant increase in the expression of glucose transporter 1 (GLUT-1), suggesting a role of oxygen in embryo development mediated through oxygen-sensitive gene expression. However, bovine embryos to at least the blastocyst stage lack a key regulator of oxygen-sensitive gene expression, hypoxia-inducible factor 1alpha (HIF1alpha). A second, less well-characterized protein (HIF2alpha) is, however, detectable from the 8-cell stage of development. Here we use differential display to determine additional gene targets in bovine embryos in response to low o..

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University of Melbourne Researchers