Journal article
New reagents for improved in vitro and in vivo examination of TGF-β signalling
RB Luwor, B Wang, TV Nheu, J Iaria, E Tsantikos, ML Hibbs, OM Sieber, HJ Zhu
Growth Factors | TAYLOR & FRANCIS LTD | Published : 2011
Abstract
Transforming growth factor-β (TGF-β) signalling controls many aspects of cell behaviour and is implicated as a key regulator in tumour formation and progression. However, evaluating levels of active TGF-β in culture medium or patient plasma and gaining definitive information regarding the activity of downstream substrates such as Sma- and Mad-related protein 3 (Smad3) in vivo with accuracy and sensitivity has been problematic. Therefore, to overcome these technical issues we have created a NIH3T3 cell line with stable pCAGA12-luc expression that can now be utilised to detect TGF-β activity with high sensitivity. In addition, we have created an adenoviral Smad3 luciferase reporter construct p..
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Funding Acknowledgements
This work was supported by grants from the National Health and Medical Research Council (NHMRC) to H-JZ. MLH is a holder of an NHMRC Research Fellowship. ET is a recipient of an Australian Post Graduate Award from the Australian Government and RBL is a recipient of the Winter and Glover Fellowship for Cancer Research from the University of Melbourne.