Journal article

Evidence for age-related and individual-specific changes in DNA methylation profile of mononuclear cells during early immune development in humans

David J Martino, Meri K Tulic, Lavinia Gordon, Megan Hodder, Tara Richman, Jessica Metcalfe, Susan L Prescott, Richard Saffery



Environment induced epigenetic effects on gene expression in early life are likely to play important roles in mediating the risk of several immune-related diseases. In order to investigate this fully, it is essential to first document temporal changes in epigenetic profile in disease-free individuals as a prelude to defining environmentally mediated changes. Mononuclear cells (MC) were collected longitudinally from a small number of females at birth, 1 year, 2.5 years and 5 years of age and examined for changes in genome-scale DNA methylation profiles using the Illumina Infinium HumanMethylation27 BeadChip array platform. MC from two males were included for comparative purposes. Flow cytomet..

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