Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting
Shunsuke Suzuki, Geoffrey Shaw, Tomoko Kaneko-Ishino, Fumitoshi Ishino, Marilyn B Renfree
BMC Evolutionary Biology | BIOMED CENTRAL LTD | Published : 2011
BACKGROUND: Genomic imprinting causes parent-of-origin specific gene expression by differential epigenetic modifications between two parental genomes. We previously reported that there is no evidence of genomic imprinting of CDKN1C in the KCNQ1 domain in the placenta of an Australian marsupial, the tammar wallaby (Macropus eugenii) whereas tammar IGF2 and H19, located adjacent to the KCNQ1 domain in eutherian mammals, are imprinted. We have now identified and characterised the marsupial orthologue of PHLDA2, another gene in the KCNQ1 domain (also known as IPL or TSSC3) that is imprinted in eutherians. In mice, Phlda2 is a dose-sensitive negative regulator of placental growth, as Cdkn1c is fo..View full abstract
Awarded by Grants-in-Aid for Scientific Research
Animals were held in our research colony under permits 1000450 and 10001910 from the Victorian Department of Sustainability and Environment. We thank Scott Brownlees, Kerry Martin and members of the wallaby research group for assistance in animal husbandry and handling. This work was supported by grants from Japan Society for the Promotion of Science (JSPS) to SS and FI, and by JSPS Postdoctoral Fellowship for Research Abroad to SS, and by an Australian Research Council Federation Fellowship to MBR.