IGFBP-2 Enhances VEGF Gene Promoter Activity and Consequent Promotion of Angiogenesis by Neuroblastoma Cells
Walid J Azar, Sheena HX Azar, Sandra Higgins, Ji-Fan Hu, Andrew R Hoffman, Donald F Newgreen, George A Werther, Vincenzo C Russo
Endocrinology | ENDOCRINE SOC | Published : 2011
IGF binding protein (IGFBP)-2 is one of the most significant genes in the signature of major aggressive cancers. Previously, we have shown that IGFBP-2 enhances proliferation and invasion of neuroblastoma cells, suggesting that IGFBP-2 activates a protumorigenic gene expression program in these cells. Gene expression profiling in human neuroblastoma SK-N-SHEP (SHEP)-BP-2 cells indicated that IGFBP-2 overexpression activated a gene expression program consistent with enhancement of tumorigenesis. Regulation was significant for genes involved in proliferation/survival, migration/adhesion, and angiogenesis, including the up-regulation of vascular endothelial growth factor (VEGF) mRNA (>2-fold). ..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This work was supported by the National Health and Medical Research Council (NHMRC) of Australia Project Grants 436955 and 1008062 (to G. A. W., V. C. R., and N.D.F.). W.J.A. is the recipient of a Melbourne University Ph.D. scholarship, and S. H. is the recipient of an NHMRC Ph.D. scholarship.