Journal article
Use of multiple reaction monitoring for multiplex analysis of colorectal cancer-associated proteins in human feces
CS Ang, J Rothacker, H Patsiouras, P Gibbs, AW Burgess, EC Nice
Electrophoresis | WILEY | Published : 2011
Abstract
Colorectal cancer (CRC) is the second most common cause of cancer-related deaths worldwide with an annual incidence of almost a million cases and an annual mortality around 500000. The fecal occult blood test is currently the first line method for CRC screening, but has unacceptably low sensitivity and specificity. Improved screening tests are therefore urgently required for early-stage CRC screening when therapy is most likely to be effective. We describe a discovery-based proteomics hypothesis using orthogonal multi-dimensional fractionation (1-D SDS-PAGE, RP-HPLC, size exclusion chromatography) to mine deep into the fecal proteome for the initial discovery process, which generated a libra..
View full abstractGrants
Awarded by Cancer Council of Victoria
Funding Acknowledgements
C.S.A. and E.C.N. were supported, in part, by research grant 433620 from the Cancer Council of Victoria and a grant from the Department of Innovation, Industry and Regional Development, Australia. The assistance of Dr. Alun Jones, University of Queensland, who helped in our initial MRM analyses, is gratefully acknowledged. This work was supported by funds from the Openational Infrastructure Support Program provided by the Victorian Government, Australia.