Journal article

Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach

Matthew J Nolan, Aaron R Jex, Jacqui A Upcroft, Peter Upcroft, Robin B Gasser

ELECTROPHORESIS | WILEY | Published : 2011

Abstract

We barcoded 25 in vitro isolates (representing 92 samples) of Giardia duodenalis from humans and other animals, which have been assembled by the Upcroft team at the Queensland Institute of Medical Research over a period of almost three decades. We used mutation scanning-coupled sequencing of loci in the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes, combined with phylogenetic analysis, to genetically characterise them. Specifically, the isolates (n514) of G. duodenalis from humans from Australia (AD113; BRIS/83/HEPU/106; BRIS/87/HEPU/713; BRIS/89/HEPU/1003; BRIS/92/HEPU/1541; BRIS/92/HEPU/1590; BRIS/92/HEPU/2443; BRIS/93/HEPU/1706), Malaysia (KL/92/IMR/1106) and Afgh..

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Grants

Awarded by National Institutes of Health, USA


Awarded by National Health and Medical Research Council of Australia


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

Funding from the Australian Research Council, Melbourne Water Corporation, The Australian Academy of Science and Fulbright Commission is gratefully acknowledged. Support from the Victorian Life Sciences Computation Initiative (VLSCI) and the IBM Collaboratory is gratefully acknowledged. This study was also supported by a U01 Cooperative Research Agreement AI75527 from the National Institutes of Health, USA, and by the National Health and Medical Research Council of Australia grant 496640. Professor Andrew Thompson and other colleagues are gratefully acknowledged for originally supplying some Giardia isolates to the Upcroft research team.