Journal article
Pharmacological response of human cardiomyocytes derived from virus-free induced pluripotent stem cells
A Mehta, YY Chung, A Ng, F Iskandar, S Atan, H Wei, G Dusting, W Sun, P Wong, W Shim
Cardiovascular Research | Published : 2011
DOI: 10.1093/cvr/cvr132
Abstract
Aims Generation of human induced pluripotent stem cell (hiPSC) lines by reprogramming of fibroblast cells with virus-free methods offers unique opportunities for translational cardiovascular medicine. The aim of the study was to reprogramme fibroblast cells to hiPSCs and to study cardiomyogenic properties and ion channel characteristics of the virus-free hiPSC-derived cardiomyocytes. Methods and resultsThe hiPSCs generated by episomal vectors generated teratomas in severe combined immunodeficient mice, readily formed embryoid bodies, and differentiated into cardiomyocytes with comparable efficiency to human embryonic stem cells. Temporal gene expression of these hiPSCs indicated that differe..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
[ "The authors acknowledge Dr Ralph Bunte, Office of Research, Duke-NUS Graduate Medical School, Singapore for his opinion on tissue pathology in the mouse teratoma assay. G.D. (Principal Research Fellow of NHMRC of Australia) was supported by a grant from the JO and JR Wicking Trust.", "This study was supported by National Research Foundation, Singapore grant, NRF2008-CRP001-68." ]