Id2 expression delineates differential checkpoints in the genetic program of CD8 alpha( ) and CD103( ) dendritic cell lineages
Jacob T Jackson, Yifang Hu, Ruijie Liu, Frederick Masson, Angela D'Amico, Sebastian Carotta, Annie Xin, Mary J Camilleri, Adele M Mount, Axel Kallies, Li Wu, Gordon K Smyth, Stephen L Nutt, Gabrielle T Belz
The EMBO Journal | WILEY | Published : 2011
Dendritic cells (DCs) have critical roles in the induction of the adaptive immune response. The transcription factors Id2, Batf3 and Irf-8 are required for many aspects of murine DC differentiation including development of CD8α(+) and CD103(+) DCs. How they regulate DC subset specification is not completely understood. Using an Id2-GFP reporter system, we show that Id2 is broadly expressed in all cDC subsets with the highest expression in CD103(+) and CD8α(+) lineages. Notably, CD103(+) DCs were the only DC able to constitutively cross-present cell-associated antigens in vitro. Irf-8 deficiency affected loss of development of virtually all conventional DCs (cDCs) while Batf3 deficiency resul..View full abstract
We thank Ken Murphy for providing the Batf3<SUP>-/-</SUP> mice; Frank Battye, Vicki Lapatis and David Baloyan for cell sorting; Elaine Major, Tara Carle and Liana Mackiewicz for expert animal care; and Lorraine O'Reilly and Sarah Oraki for technical advice. This work was supported by grants and fellowships from the National Health and Medical Research Council of Australia (GTB, SLN, LW and GKS), the Australian Research Council Future Fellowship (AK), Swiss National Science Foundation (FM), the Sylvia and Charles Viertel Foundation and the Howard Hughes Medical Institute (GTB) and the Pfizer Australia Research Fellowship (SLN).