A Genome-Wide Survey of Imprinted Genes in Rice Seeds Reveals Imprinting Primarily Occurs in the Endosperm
Ming Luo, Jennifer M Taylor, Andrew Spriggs, Hongyu Zhang, Xianjun Wu, Scott Russell, Mohan Singh, Anna Koltunow
PLoS Genetics | PUBLIC LIBRARY SCIENCE | Published : 2011
Genomic imprinting causes the expression of an allele depending on its parental origin. In plants, most imprinted genes have been identified in Arabidopsis endosperm, a transient structure consumed by the embryo during seed formation. We identified imprinted genes in rice seed where both the endosperm and embryo are present at seed maturity. RNA was extracted from embryos and endosperm of seeds obtained from reciprocal crosses between two subspecies Nipponbare (Japonica rice) and 93-11 (Indica rice). Sequenced reads from cDNA libraries were aligned to their respective parental genomes using single-nucleotide polymorphisms (SNPs). Reads across SNPs enabled derivation of parental expression bi..View full abstract
This project was funded by an Acorn Grant to ML, a DIISR India-Australia Grant to AK, and support from CSIRO's Transformation Biology initiative. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.