Journal article

A Genome-Wide Survey of Imprinted Genes in Rice Seeds Reveals Imprinting Primarily Occurs in the Endosperm

Ming Luo, Jennifer M Taylor, Andrew Spriggs, Hongyu Zhang, Xianjun Wu, Scott Russell, Mohan Singh, Anna Koltunow

PLoS Genetics | PUBLIC LIBRARY SCIENCE | Published : 2011

Abstract

Genomic imprinting causes the expression of an allele depending on its parental origin. In plants, most imprinted genes have been identified in Arabidopsis endosperm, a transient structure consumed by the embryo during seed formation. We identified imprinted genes in rice seed where both the endosperm and embryo are present at seed maturity. RNA was extracted from embryos and endosperm of seeds obtained from reciprocal crosses between two subspecies Nipponbare (Japonica rice) and 93-11 (Indica rice). Sequenced reads from cDNA libraries were aligned to their respective parental genomes using single-nucleotide polymorphisms (SNPs). Reads across SNPs enabled derivation of parental expression bi..

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University of Melbourne Researchers