Neural Development in Human Embryonic Stem Cells-Applications of Lentiviral Vectors
Mirella Dottori, Cheryl Tay, Stephanie M Hughes
JOURNAL OF CELLULAR BIOCHEMISTRY | WILEY | Published : 2011
The derivation of neural lineages from human embryonic stem cells (hESCs) in vitro is based largely on exposure of hESCs to exogenous signals and substrates, designed to mimic conditions in the developing embryo. However, selection of specific lineages and the discovery of gene function in human neural development may be enhanced by the ability to intrinsically regulate gene expression. Recombinant lentiviral vectors provide an efficient method to stably introduce genes into hESC and their differentiating derivatives. Here we review the methods used to derive neural cells from hESCs, transduction of these cells with lentiviral vectors, and improvements that have been made to the vectors to e..View full abstract
Awarded by Royal Society of New Zealand
Grant sponsor: Royal Society of New Zealand Marsden Fund; Grant number: UOO0412.