Analysis of the Mitogen-activated protein kinase kinase 4 (MAP2K4) tumor suppressor gene in ovarian cancer
Sally J Davis, David YH Choong, Manasa Ramakrishna, Georgina L Ryland, Ian G Campbell, Kylie L Gorringe
BMC Cancer | BMC | Published : 2011
BACKGROUND: MAP2K4 is a putative tumor and metastasis suppressor gene frequently found to be deleted in various cancer types. We aimed to conduct a comprehensive analysis of this gene to assess its involvement in ovarian cancer. METHODS: We screened for mutations in MAP2K4 using High Resolution Melt analysis of 149 primary ovarian tumors and methylation at the promoter using Methylation-Specific Single-Stranded Conformation Polymorphism analysis of 39 tumors. We also considered the clinical impact of changes in MAP2K4 using publicly available expression and copy number array data. Finally, we used siRNA to measure the effect of reducing MAP2K4 expression in cell lines. RESULTS: In addition t..View full abstract
This study was funded by the Victorian Breast Cancer Research Consortium, Australia (VBCRC) and the Australian National Health and Medical Research Council (NHMRC). MR is supported by a Cancer Council of Victoria Postgraduate Scholarship. GLR is supported by an Australian Postgraduate Award.