Manipulation of Estrogen Synthesis Alters MIR202*Expression in Embryonic Chicken Gonads
Stephanie C Bannister, Craig A Smith, Kelly N Roeszler, Timothy J Doran, Andrew H Sinclair, Mark LV Tizard
Biology of Reproduction | OXFORD UNIV PRESS INC | Published : 2011
Tissue-specific patterns of microRNA (miRNA) expression contribute to organogenesis during embryonic development. Using the embryonic chicken gonads as a model for vertebrate gonadogenesis, we previously reported that miRNAs are expressed in a sexually dimorphic manner during gonadal sex differentiation. Being male biased, we hypothesised that up-regulation of microRNA 202* (MIR202*) is characteristic of testicular differentiation. To address this hypothesis, we used estrogen modulation to induce gonadal sex reversal in embryonic chicken gonads and analyzed changes in MIR202* expression. In ovo injection of estradiol-17beta at Embryonic Day 4.5 (E4.5) caused feminization of male gonads at E9..View full abstract
Supported by Australian Research Council (ARC) Discovery Grant (C. A. S. and A. H. S.) and CSIRO Livestock Industries Project Funding (M. L. V. T. and T.J.D.). S. C. B. is supported by an Australian Postgraduate Award (APA) scholarship from the University of Melbourne and CSIRO Livestock Industries PhD Top-up Scholarship (CSIRO).