Journal article
Analysis of Brca1-deficient mouse mammary glands reveals reciprocal regulation of Brca1 and c-kit
CE Smart, A Wronski, JD French, SL Edwards, ML Asselin-Labat, N Waddell, K Peters, BL Brewster, K Brooks, K Simpson, N Manning, SR Lakhani, S Grimmond, GJ Lindeman, JE Visvader, MA Brown
Oncogene | Published : 2011
DOI: 10.1038/onc.2010.538
Abstract
Disruption of the breast cancer susceptibility gene Brca1 results in defective lobular-alveolar development in the mammary gland and a predisposition to breast tumourigenesis in humans and in mice. Recent evidence suggests that BRCA1 loss in humans is associated with an expansion of the luminal progenitor cell compartment in the normal breast and tumours with a luminal progenitor-like expression profile. To further investigate the role of BRCA1 in the mammary gland, we examined the consequences of Brca1 loss in mouse mammary epithelial cells in vitro and in vivo. Here, we show that Brca1 loss is associated with defective morphogenesis of SCp2 and HC11 mouse mammary epithelial cell lines and ..
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Funding Acknowledgements
We are grateful to Bob Simpson for advice on real-time PCR, Kym Kelly-Taylor for animal husbandry, Vani Selvarajan for mouse genotyping and Lynne Reid for cryosectioning. We acknowledge the ARC Special Research Centre for Functional and Applied Genomics at The Institute of Molecular Bioscience, University of Queensland for support with microarray profiling and analysis. This work was supported by a National Breast Cancer Foundation of Australia and The University of Queensland. JF was supported by an NBCF Training fellowship, SE was supported by an NHMRC CJ Martin Fellowship and JV, GL and SG are Research Fellows of the National Health and Medical Research Council of Australia.