Journal article
Relationship between human intestinal dendritic cells, gut microbiota, and disease activity in Crohn's disease
SC Ng, JL Benjamin, NE McCarthy, CRH Hedin, A Koutsoumpas, S Plamondon, CL Price, AL Hart, MA Kamm, A Forbes, SC Knight, JO Lindsay, K Whelan, AJ Stagg
Inflammatory Bowel Diseases | WILEY-BLACKWELL | Published : 2011
DOI: 10.1002/ibd.21590
Abstract
Background: Altered intestinal dendritic cell (DC) function underlies dysregulated T-cell responses to bacteria in Crohn's disease (CD) but it is unclear whether composition of the intestinal microbiota impacts local DC function. We assessed the relationship between DC function with disease activity and intestinal microbiota in patients with CD. Methods: Surface expression of Toll-like receptor (TLR)-2, TLR-4, and spontaneous intracellular interleukin (IL)-10, IL-12p40, IL-6 production by freshly isolated DC were analyzed by multicolor flow cytometry of cells extracted from rectal tissue of 10 controls and 28 CD patients. Myeloid DC were identified as CD11c +HLA-DR +lin-/dim cells (lin = ant..
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Funding Acknowledgements
Funded by the Broad Foundation.We acknowledge the support of the National funding from National Institute of Health Research via the comprehensive biomedical research centers based respectively at Guy's and St Thomas' Hospital for King's College London and at University College Hospital for University College of London. We also acknowledge the help of Cynthia Cheung, Chinese University of Hong Kong for figure production.