Genome-wide analysis distinguishes hyperglycemia regulated epigenetic signatures of primary vascular cells
Luciano Pirola, Aneta Balcerczyk, Richard W Tothill, Izhak Haviv, Antony Kaspi, Sebastian Lunke, Mark Ziemann, Tom Karagiannis, Stephen Tonna, Adam Kowalczyk, Bryan Beresford-Smith, Geoff Macintyre, Kelong Ma, Hongyu Zhang, Jingde Zhu, Assam El-Osta
Genome Research | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2011
Emerging evidence suggests that poor glycemic control mediates post-translational modifications to the H3 histone tail. We are only beginning to understand the dynamic role of some of the diverse epigenetic changes mediated by hyperglycemia at single loci, yet elevated glucose levels are thought to regulate genome-wide changes, and this still remains poorly understood. In this article we describe genome-wide histone H3K9/K14 hyperacetylation and DNA methylation maps conferred by hyperglycemia in primary human vascular cells. Chromatin immunoprecipitation (ChIP) as well as CpG methylation (CpG) assays, followed by massive parallel sequencing (ChIP-seq and CpG-seq) identified unique hyperacety..View full abstract
We acknowledge grant and fellowship support from the Juvenile Diabetes Research Foundation International (JDRF), the Diabetes Australia Research Trust (DART), the National Health and Medical Research Council (NHMRC), the National Heart Foundation of Australia (NHF), the INSERM-NHMRC Cooperation Programme, the Foundation for Polish Science (FNP), and the National Research Program for Basic Research of China. Supported in part by the Victorian Government's Operational Infrastructure Support Program.