Journal article
Exploring the Fasciola hepatica tegument proteome
RA Wilson, JM Wright, W de Castro-Borges, SJ Parker-Manuel, AA Dowle, PD Ashton, ND Young, RB Gasser, TW Spithill
International Journal for Parasitology | ELSEVIER SCI LTD | Published : 2011
Abstract
The surface tegument of the liver fluke Fasciola hepatica is a syncytial cytoplasmic layer bounded externally by a plasma membrane and covered by a glycocalyx, which constitutes the interface between the parasite and its ruminant host. The tegument's interaction with the immune system during the fluke's protracted migration from the gut lumen through the peritoneal cavity and liver parenchyma to the lumen of the bile duct, plays a key role in the fluke's establishment or elimination. However, little is known about proteins of the tegument surface or its secretions. We applied techniques developed for the blood fluke, Schistosoma mansoni, to enrich a tegument surface membrane preparation and ..
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Funding Acknowledgements
This research was supported by a Researcher Exchange award from the ARC/NHMRC Research Network for Parasitology, Australia and the Australian Society for Parasitology, and with funds from Charles Sturt University and La Trobe University, Australia. We acknowledge the assistance of Dr. Naveed Aziz and Ms. Celina Whalley of the Genomics Laboratory, Technology Facility, Department of Biology, University of York, UK, in performing the Roche454 sequencing of F. hepatica transcripts that were assembled into the contigs and singlets comprising the FhB dataset. Current research in the Gasser Laboratory is supported mainly through grants from the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) and Melbourne Water Corporation, Australia. Support from the Victorian Life Sciences Computation Initiative (VLSCI) and the IBM 'Collaboratory' is gratefully acknowledged (RBG).