Journal article
Evolution of multidrug resistance during staphylococcus aureus infection involves mutation of the essential two component regulator WalKR
BP Howden, CRE McEvoy, DL Allen, K Chua, W Gao, PF Harrison, J Bell, G Coombs, V Bennett-Wood, JL Porter, R Robins-Browne, JK Davies, T Seemann, TP Stinear
Plos Pathogens | Published : 2011
Open access
Abstract
Antimicrobial resistance in Staphylococcus aureus is a major public health threat, compounded by emergence of strains with resistance to vancomycin and daptomycin, both last line antimicrobials. Here we have performed high throughput DNA sequencing and comparative genomics for five clinical pairs of vancomycin-susceptible (VSSA) and vancomycin-intermediate ST239 S. aureus (VISA); each pair isolated before and after vancomycin treatment failure. These comparisons revealed a frequent pattern of mutation among the VISA strains within the essential walKR two-component regulatory locus involved in control of cell wall metabolism. We then conducted bi-directional allelic exchange experiments in ou..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was funded by the National Health and Medical Research Council (NHMRC), Australia (grant number 628652). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.