Journal article
Systemic upregulation of NADPH oxidase in diet-induced obesity in rats
F Jiang, HK Lim, MJ Morris, L Prior, E Velkoska, X Wu, GJ Dusting
Redox Report | Published : 2011
Abstract
Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is upregulated in a variety of tissues in obesity. It is still unclear as to whether NADPH oxidase upregulation in a specific tissue is part of a systemic response. Here we analyzed the expression pattern of NADPH oxidase in vascular, adipose, and kidney tissues in a rat model of diet-induced obesity. After weaning, rats were fed either a normal or highfat diet for 12 weeks. The high-fat diet resulted in 20% increased body weight. In the aorta, Nox4 expression was increased by three-fold in obese rats. Upregulations of p22phox and p47phox in adipose, and Nox4, p22phox, and p47phox in kidney were observed in obesity. Marked increases..
View full abstractGrants
Awarded by National 973 Basic Research Program of China
Awarded by NSFC
Funding Acknowledgements
The authors thank Nancy Guo for technical assistance in the real-time PCR assay. This study was partially supported by Project Grants from the National Health and Medical Research Council (NHMRC) of Australia, Grants-in-Aid from the National Heart Foundation of Australia, National 973 Basic Research Program of China (2010CB732605), and NSFC (grant 81070164). G.J.D. receives an NHMRC Principal Research Fellowship.