Journal article

A Bedside Assay to Detect Streptococcus pneumoniae in Children With Empyema

Roxanne E Strachan, Anita Cornelius, Gwendolyn L Gilbert, Tanya Gulliver, Andrew Martin, Tim McDonald, Gillian M Nixon, Rob Roseby, Sarath Ranganathan, Hiran Selvadurai, Greg Smith, Manuel Soto-Martinez, Sadasivam Suresh, Laurel Teoh, Kiran Thapa, Claire E Wainwright, Adam Jaffe

Pediatric Pulmonology | WILEY | Published : 2011


BACKGROUND: Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. AIMS: To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema. METHODS: Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid. RESULTS: S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) ..

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