Journal article

Widespread pyrethroid resistance in Australian diamondback moth, Plutella xylostella (L.), is related to multiple mutations in the para sodium channel gene

NM Endersby, K Viduka, SW Baxter, J Saw, DG Heckel, SW McKechnie



Populations of Plutella xylostella, extending over 3800 km in southern Australia, show no genetic structure as assessed by microsatellite markers; yet outbreaks of pyrethroid resistance occur sporadically in cropping areas. Since mutations in the para voltage-gated sodium channel gene have been implicated in pyrethroid resistance, we looked for DNA sequence variation at this target among Australian moths. We found two resistance mutations previously reported for this species (L1014F and T929I), as well as a novel substitution (F1020S). Of the eight possible haplotypes formed by combinations of these three biallelic polymorphisms, only four were found in Australian populations: the wild-type ..

View full abstract


Awarded by Biotechnology and Biological Sciences Research Council

Funding Acknowledgements

Thanks to the Department of Agriculture, Western Australia and Queensland Department of Primary Industries for contributing some P. xylostella for this study. Thanks to Philip Batterham for use of CESAR Melbourne facilities in the latter part of this study. The study was funded by an Australian Research Council Strategic Partnership with Industry Research & Training Grant, Department of Primary Industries Victoria (Industry Partner), the Grains Research and Development Corporation, CESAR-a Special Research Centre of the Australian Research Council, Biotechnology and Biological Sciences Research Council (BBSRC) and the Max-Planck Gesellschaft.